CAS No.: 77591-33-4
Molecular Formula: C212H350N56O78S
Molecular Weight: 4963.49
Purity (HPLC): 95%min
Single Impurity (HPLC): 2.0%max
Amino Acid Composition: ± 10% of theoretical
Peptide Content (N%): ≥ 80.0%
Water Content(Karl Fischer): ≤ 5.0%
Acetate Content (HPIC): ≤ 15.0%
MS(ESI): Consistent
Mass Balance: 95.0~105.0%
Appearance: White powder
TB-500 is a synthetic fraction of the protein thymosin beta-4, which is present in virtually all human and animal cells. The main purpose of this peptide is to promote healing. It also promotes creation of new blood and muscle cells. The healing effects of TB-500 have been observed in tendons, ligaments, muscle, skin, heart, and the eyes. Thymosin beta-4 is naturally produced in higher concentration where tissue has been damaged. This peptide is also a very potent anti-inflamatory agent.
TB-500 is a synthetic version of the naturally occurring peptide present in virtually all human and animal cells, Thymosin Beta-4. This potent peptide is a member of a ubiquitous family of 16 related molecules with a high conservation of sequence and localization in most tissues and circulating cells in the body. TB-500 not only binds to actin, but also blocks actin polymerization and is the actin-sequestering molecule in eukaryotic cells.
TB-500 was identified as a gene that was up-regulated four-to-six fold during early blood vessel formation and found to promote the growth of new blood cells from the existing vessels. This peptide is present in wound fluid and when administered subcutaneously, it promotes wound healing, muscle building and speeds up recovery time of muscles fibres and their cells. An additional key factor of TB-500 is that it promotes cell migration through a specific interaction with actin in the cell cytoskeleton. It has been demonstrated that a central small amino acid long-actin binding domain has both blood cell reproduction and wound healing characteristics. These characteristics are uncovered by accelerating the migration of endothelial cells and keratinocytes. It also increases the production of extracellular matrix-degrading enzymes.
• Endothelial (blood vessels) cell differentiation
• Angiogenesis (growth of new blood cells from pre-existing vessels) in dermal tissues
• Keratinocyte migration
• Collagen deposition
• Decreases inflammation of tissue in joints
• Increase muscle growth with huge increases in endurance and strength noted
• Relaxed muscle spasm and improved muscle tone
• Increase the exchange of substance between cells
• Encourage tissue repair
• Stretches connective tissue and helps maintain flexibility
• Prevents the formations of adhesions and fibrous bands in muscles, tendons and ligaments.
TB-500 is typically provided as lyophilized (freeze-dried) powder in vials of 2.0 mg. A convenient amount of sterile or bacteriostatic water will be added, such as 1.0 mL. In the most common dosing protocol, the entire vial is taken at one time. If taking the entire vial, the entire amount will be drawn into a syringe, typically an insulin syringe, and injected. Injection may be subcutaneous, intramuscular, or intravenous, according to personal preference.
The most common dosing protocol is to take 2.0 or 2.5 mg of TB-500 twice per week for four to six weeks, and then reduce to a lower dosing rate such as once or twice per month for maintenance.
The body of knowledge is as yet limited with regard to TB-500 dosage. The above protocol may or may not be the most efficient, but it is the protocol with the most track record. It’s entirely possible that as knowledge is gained, method of use will change. A reasonable possibility, for example, is to increase dosing frequency to 3x/week (which I have done personally to good effect), or to increase dosing to 4 or 5 mg twice per week. Before going to such a program, however, I recommend trying the standard protocol first.
TB-500 has been known to promote at least partial regrowth of hair which has been lost to male-pattern baldness, to darken at least a portion of hair which has grayed, and is reported to enhance muscle mass in horses.
The latter effect may be due to promoting differentiation of satellite cells. Bodybuilders who have used TB-500 for healing purposes have however generally not reported such an effect. However, it might be that the effect occurred, but was attributed to other causes, or that effect did not occur because TB-500 is generally used only when injured, which tends not to be a time of training for best gains.
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